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Slide 233 of 314
|Isolation of Genomic DNA |
|Human cells contain large amounts of genomic DNA which constitute the chromosomes of each cell. This is distinct from mitochondrial DNA which is a small circular DNA molecule similar to plasmid DNA and, therefore, isolated by different methods. |
|Isolation of genomic DNA is very easy. Cells are first lysed in a test tube and the DNA is extracted from the lysate through phenol / chloroform organic extraction. Proteins bound to the DNA are denatured by the organic solvent. DNA remains in aqueous solution. The aqueous and organic phases separate by centrifugation. The aqueous phase is removed with the DNA. The DNA is precipitated out of solution with alcohol and salt. The pellet of DNA at the bottom of the test tube may then be dissolved in a buffer and used for further experimentation. |